Procedure
1. Pour 5 mL of peroxide into each of the three test tubes.
2. Allow one test tube to rest in the test tube rack to maintain room temperature. Place one test tube into a large beaker filled with ice. Place the remaining test tube into a beaker with water on a hot plate.
3. Place five enzyme discs into a erlenmeyer flask and pour the peroxide of the test tube that was previously on the test tube rack into the erlenmeyer flask through a funnel. Shake the mixture for 30 seconds and calculate the amount of gas produced by the change in water level.
4. Repeat step three with the other two test tubes after the following conditions have been met: When the peroxide within the beaker of ice has reached a temperature of 6°C and when the peroxide within the beaker of water on the hot plate has reached a temperature of 60°C.
Results
Test tube at room temperature: 570 mL of gas produced.
Test tube at 6°C: 100
Test tube at 60°C: 200
